Fig 5. Effect of in vivo prophylactic fingolimod (0.3 mg/kg) on the depolarization-evoked exocytosis of endogenous GABA from nerve terminals isolated from selected regions of the CNS of control and EAE mice.

Female mice were randomly assigned to the following groups: control mice (empty bar, n = 8 mice); fingolimod-treated control mice (rising-right hatched empty bar, n = 8 mice); EAE mice (grey bar, n = 8 mice); fingolimod-treated EAE mice (rising-right hatched grey bar, n = 8 mice). Fingolimod was administered as described above. At 21 ± 1 d.p.i. mice were sacrificed and cortical (A), hippocampal (B) and spinal cord (C) synaptosomes were isolated to monitor the exocytosis of endogenous GABA elicited by a mild depolarizing stimulus as previously described. Results are expressed as KCl-evoked overflow; data are expressed as pmoles / mg protein and represent the mean ± SEM. * p < 0.05 at least versus control untreated mice; ° p < 0.05 at least versus EAE untreated mice.