Fig 2. The effect of pH on condensed tannin (CT) efficacy to aggregate BSA.

ER₅₀ is the half maximal effective ratio (values were corrected for CT content): 7.3 (±0.2) at pH 3, 1.0 (±0.0) at pH 4, 2.1 (±0.0) at pH 5, 3.4 (±0.0) at pH 6 (for citrate and BIS-TRIS buffer), 9.8 (±0.3) at pH 7, and no aggregation was observed at pH 8. The values in parentheses and error bars indicate the estimated error of the fit of the titration data for ER₅₀ (after averaging experimental data points, typically n = 3 replicates).