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. 2017 Jan 26;12(1):e0170674. doi: 10.1371/journal.pone.0170674

Fig 4. Activity of Alkaline phosphatase of cells exposed to fluoride with and without SB431542.

Fig 4

Cells were treated with 1 mg/L, 4 mg/L, and 16 mg/L of fluoride with or without 10μmol/L SB431542 for 7 days. The deposition of Cobalt sulfide particles was stained into light black granules in cytoplasm. The positive staining for deposition of Cobalt sulfide particles was shown in control group (a), 1 mg F−/L group (b), 4 mg F−/L (c), 16 mg F−/L group (d), SB431542 control group (e), SB431542 +1 mg F−/L group (f), SB431542 +4 mg F−/L (g), SB431542 + 16 mg F−/L group (h), TGF-β1control group (i), TGF-β1 + 1 mg F−/L group (j), TGF-β1 +4 mg F−/L (k), TGF-β1 + 16 mg F−/L group (l) under microscopy (red arrowheads; scale bar, 50 μm).