Fig 5. Expression of Runx2, Smad3 and ALK5 in cells BMSC exposed to fluoride with and without SB431542.

The BMSCs were collected and extracted mRNA by Trizol reagent. Realtime PCR was used to analyze Runx2, Smad3 and ALK5 expression. The GAPDH was used as inner control. Results are expressed as mean± SD (n = 3). (*P < 0.05, **P < 0.01 compare with control group; aa P < 0.01, compare with two groups).