Figure 2.
Measurement of bacterial burden using in vivo bioluminescence. S. aureus possessing the bioluminescent construct in a stable plasmid (Xen36) in three inoculums (1 × 102, 1 × 103, 1 × 104 CFU) or no bacteria as a control (n =6 mice per group) were inoculated into the L4 spinous process of mice in the presence of a stainless steel implant. (A) Bacterial counts as measured by in vivo S. aureus bioluminescence (mean maximum flux [photons/s/cm2/sr] ± sem [logarithmic scale]). (B) Representative in vivo S. aureus bioluminescence on a color scale overlaid on top of a grayscale image of mice.