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. 2017 Jan 26;12(1):e0170419. doi: 10.1371/journal.pone.0170419

Fig 4. Effect of N-linked glycosylation on NTCP protein expression.

Fig 4

(A and B) HA-tagged NTCP was immunoprecipitated (IP) from lysates of HepG2 cells stably expressing NTCP-WT, NTCP-N5Q, NTCP-N11Q or NTCP-N5,11Q. IP samples were subjected to immunoblotting for NTCP (using anti-HA-hrp). (B) IP samples were digested with PNGase F for 1h at 37°C (500 units) prior to immunoblotting for NTCP. (C) NTCP was quantified by image J and expressed relative to NTCP-WT. Molecular mass is given in kDa on the left-hand side. Results are mean +/- sd. *Indicates significant different from NTCP-WT (p <0.05).