Fig 2. Cardiac macrophage abundance during pressure-overload remodeling.

A, Representative gating strategy used to identify cardiac macrophages, as well as CD206⁺ and CD206⁻ macrophage subsets, in mouse hearts. B, Quantitation of flow cytometry data collected from cardiac mononuclear cell isolates at the indicated time points after TAC or sham operation, including total CD45⁺ leukocytes, total CD45⁺CD11b⁺F480⁺MHCII⁺ macrophages, and C, CD206⁻ (M1) and CD206⁺ (M2) macrophages. D, Quantitation of flow cytometry data for Lin^–CD11b⁺Ly6C^hi and Lin^–CD11b⁺Ly6C^low peripheral blood monocytes at the indicated time points. n = 6–10 in sham groups; n = 6–12 in TAC groups. *p<0.05 vs. sham.