. 2016 Dec 30;5:e23156. doi: 10.7554/eLife.23156

Table 2.

Primers. Oligonucleotide sequences are written 5 $^{'}$ to 3 $^{'}$ . Bold sequences indicate variable regions. The ‘1H library’ and ‘3H library’ primers respectively contained the 1H and 3H variable regions (bold) analyzed in this paper. These primer libraries were synthesized by LC Biosciences using microarray-based DNA synthesis. All other primers were ordered from Integrated DNA Technologies. The ‘[XX]’ portion of L1AF_XX and L1AR_XX indicates the location of each of 64 different barcodes (i.e., XX = 01, 02, $\dots$ , 64), which ranged in length from 7 bp to 10 bp and which differed from each other by at least two substitution mutations.

DOI: http://dx.doi.org/10.7554/eLife.23156.019

Name	Sequence
`1H library`	`GTGTTGCCTCTGGATTCACTTTTAGTGACTACTGGATGAACTGGGTCCGCCAGTCTCCAGA`
`3H library`	`GTGACTGAGGTTCCTTGACCCCAGTAGTCCATACCATAGTAAGAACCCGTACAGTAATAGATACCCAT`
`oRAL10`	`TTCTGAGGAGACGGTGACTGAGGTTCCTTG`
`oRAR10`	`TGAAGACATGGGTATCTATTACTGTACG`
`oRAL11`	`CAGTCCTTTCTCTGGAGACTGGCG`
`oRAR11`	`ATGAAACTCTCCTGTGTTGCCTCTGGATTC`
`3H1F`	`TTCTGAGGAGACGGTGACT`
`3H2R`	`TGAAGACATGGGTATCTATTACTGTAC`
`1H2F`	`CAGTCCTTTCTCTGGAGACTG`
`1H1R`	`ATGAAACTCTCCTGTGTTGCCT`
`oRA10`	`GCATATCTAAGGTCTCGTTCTGAGGAGACGGTGAC`
`oRA11`	`GCCGATTGTTGGTCTCCATGAAACTCTCCTGTGTTGC`
`PE1v3ext`	`AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG`
`PE2v3`	`AAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCT`
`L1AF_XX`	`ACACTCTTTCCCTACACGACGCTCTTCCGATCT[XX]AGTCTTCTTCAGAAATAAGC`
`L1AR_XX`	`CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT[XX]GCTTGGTGCAACCTG`