Table 1. Sequences of primers used in the SnM-PCR and in the 18S rDNA PCR for genera Plasmodium.
| PCR | Primer | Sequence | Specificity | Product Size | Reference |
|---|---|---|---|---|---|
| SnM-PCR First round | PLF | 5’-AGTGTGTATCCAATCGAGTTTC-3’ | Plasmodium | 783-821bp* | [17] |
| HUF | 5’-GAGCCGCCTGGATACCGC-3’ | Mammals | 231 bp++ | ||
| UNR | 5’-GACGGTATCTGATCGTCTTC-3’ | Universal | |||
| SnM-PCR Second round | PLF | 5’-AGTGTGTATCCAATCGAGTTTC-3’ | Plasmodium | ||
| MAR | 5’-GCCCTCCAATTGCCTTCTG-3’ | P. malariae | 269 bp | ||
| FAR | 5’-AGTTCCCCTAGAATAGTTACA-3’ | P. falciparum | 369 bp | ||
| VIR | 5’-AGGACTTCCAAGCCGAAGC-3’ | P. vivax | 499 bp | ||
| 18S rRNA PCR | rPLU5 | 5’-CCTGTTTGTTGCCTTAAACTTC-3’ | Plasmodium | 1050 bp | [18] |
| rPLU6 | 5’-TTAAAATTGTTGCAGTTAAAACG-3’ | Plasmodium |
*Size depending on the species, P. malariae (821 bp), P. falciparum (787 bp), P. vivax (783 bp).
++ In the first reaction, the UNR-HUF primers form a product of 231 bp which is used as an individual positive control, and must be present in all the samples except the negative control.