Table 1. Pm activity against S-2251, free and in complex with rSK variants.
| Pm in complex with | kcat s-1 (SEM) | KM μM (SEM) | kcat/ KM mM-1s-1 (SEM) |
|---|---|---|---|
| Pm alone | 25.5 (± 1.9) | 368 (± 10) | 69.3 (± 2.0) |
| rSK-H46A | 60.4 (± 3.8) | 361 (± 10) | 170 (± 4.2) |
| rSK-M1GAS | 26.7 (± 2.4) | 156 (± 60) | 171 (± 10.5) |
| rSK-H46A del 1–59 | 25.1 (± 2.0) | 377 (± 20) | 66.6 (± 2.4) |
| rSK-M1GAS del 1–59 | 25.6 (± 1.6) | 426 (± 20) | 64.4 (± 2.0) |
| Pm + Fgn | 14.5 (± 4.2) | 1600 (1003) | 9.13 (10.0) |
| rSK-H46A + Fgn | 70.3 (± 5.7) | 400 (± 10) | 160 (± 6.0) |
| rSK-M1GAS + Fgn | 64.7 (± 12.6) | 400 (± 70) | 159 (± 16.9) |