Fig 2. CDCA repressed AKR1D1 expression in time and dose-dependent manners in HepG2 cells.

(A) HepG2 cells were treated with either CDCA (25 μM) or vehicle DMSO for 8, 24 or 30 hrs, followed by detection of AKR1D1 mRNA with real-time PCR. (B) HepG2 cells were treated with various concentrations of CDCA (0, 5, 25, 50, 100 μM) for 30 hrs, followed by detection of AKR1D1 mRNA expression with real-time PCR. **p<0.01 with the Student’s t-test for pair-wise comparison.