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. 2017 Jan 26;12(1):e0170960. doi: 10.1371/journal.pone.0170960

Fig 3. Akr1d1 expression was severely repressed by CDCA in vivo in mice.

Fig 3

Two groups of mice (n = 6/group) were treated with CDCA (5mg/kg) or vehicle propanediol through intraperitoneal injection twice a day for 3 days. Twelve hours after the last injection, mice were euthanized and liver tissues were harvested and processed for mRNA and protein analyses. (A) the effects of CDCA treatment on Akr1d1, (B) Cyp7a1 and (C) Cyp8b1 mRNA levels detected by real-time PCR. (D) the effects of CDCA treatment on Akr1d1, Cyp7a1 and Cyp8b1 protein expression detected by Western blotting, and (E) quantification of Akr1d1, (F) Cyp7a1 and (G) Cyp8b1 protein levels in (). The data are presented as mean ± SD of the groups. The Student’s t-test was applied to pair-wise comparison. *p<0.05, **p<0.01.