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. 2017 Jan 26;12(1):e0170960. doi: 10.1371/journal.pone.0170960

Fig 7. FXR signaling was not involved in regulating AKR1D1 by bile acids in HepG2 cells.

Fig 7

(A) HepG2 cells were treated with FXR agonist GW4064 (1μM) or vehicle DMSO (0.1%) for 30h, followed by detection of AKR1D1 and BSEP mRNA levels by real-time PCR. (B) HepG2 cells were transfected with FXRα1, FXRα2 or vector, followed by treatment with GW4064 (1μM) for 30h. The expression levels of AKR1D1 and BSEP were detected by real-time PCR. The Student’s t-test was applied to pair-wise comparison. One-way ANOVA was applied to analyze data with multiple groups, followed by Tukey post-hoc test for multiple comparisons. ** p<0.01.