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. 2017 Jan 26;12(1):e0170960. doi: 10.1371/journal.pone.0170960

Fig 8. FXR signaling was not involved in regulating AKR1D1 by bile acids in vivo in mice.

Fig 8

(A) two groups of mice (n = 6/group) were treated with GW4064 (5mg/kg) or vehicle propanediol through intraperitoneal injection twice a day for 3 days. The expression levels of Akr1d1 and Bsep were quantified by real-time PCR and (B) Western blot. (C) quantification of Akr1d1 and (D) Bsep protein levels in (B). (E) the expression levels of Akr1d1 and Bsep in wt and FXR-knockout (FXR-/-) mice were determined by real-time PCR and (F) Western blot. (G) quantification of Akr1d1 and (H) Bsep protein levels in (F). The data are presented as mean ± SD of the groups of mice. The Student’s t-test was applied to pair-wise comparison. ** p<0.01.