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. 2017 Jan 26;12(1):e0170960. doi: 10.1371/journal.pone.0170960

Fig 10. Inhibition of MAPK/JNK signaling pathway abolished CDCA-mediated regulation of AKR1D1.

Fig 10

(A) HepG2 cells were treated with CDCA (25μM) in the absence or presence of MAPK/JNK inhibitor SP600125 (1μM), MAPK/ERK1/2 inhibitor PD98059 (5μM) or vehicle for 30 hrs, followed by detection of AKR1D1 mRNA by real-time PCR and (B) AKR1D1 protein by Western blot. (C) quantification of AKR1D1 protein levels in (B). One-way ANOVA was applied to analyze the data, followed by Tukey post-hoc test for multiple comparisons. * p<0.05 and ** p<0.01.