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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Biotechnol Bioeng. 2016 Jun 9;113(12):2686–2697. doi: 10.1002/bit.26022

Figure 4.

Figure 4

Localization of H3-targeted polyplexes within the nucleus at five different time points following H3-targeted (a-d), untargeted (e), and sH3 (f) polyplex transfection in MCF10A (light gray) and IBC SUM149 (dark gray) cells. (a-c) Representative confocal microscopy z-slice images of IBC cells with the nuclei stained with DAPI (4',6-diamidino-2-phenylindole) (blue) following a pulse transfection with H3-targeted PEI polyplexes (red) (a) 1 h, (b) 4 h, and (c) 11 h post-transfection. The scale bar (shown in a) = 5 μm. The cell borders were outlined in white by comparison with the corresponding phase images by using Zen software. (d-f) Quantification of colocalization from confocal microscopy images was performed with Volocity Image Analysis software. Each data point represents the mean ± SE for a minimum of 100 polyplexes, with ~10 images analyzed per colocalization replicate. Gray box indicates mitosis. * indicates statistically significant difference between cell line for given time point (P < 0.05). ** indicates statistically significant difference between previous time point for given cell line (P < 0.05).