Inflammatory cytokines, IFN-γ and TNF-α, were measured by intracellular staining from human whole blood after activation with PMA-Ionomycin in the presence or absence of Y27632 at several concentrations. In the presence of Y27632, cytokine levels from (A) CD4+ and (B) CD8+ T cells were significantly decreased. Data expressed as mean ± SD (*p<0.05, **p<0.01, ns = not significant, n=3).