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. Author manuscript; available in PMC: 2017 Jun 24.
Published in final edited form as: Q Rev Biophys. 2016 Jun 24;49:e10. doi: 10.1017/S003358351600007X

Figure 7.

Figure 7

Under both in vitro (right, grey) and in vivo-like conditions with molecular crowding (left, pink) RNA fold into their native state that is functional, indicated in this figure by catalysis. High concentrations of Mg2+ (10 mM or higher) is needed to achieve the folded state in vitro compared to in vivo-like crowded conditions where low physiological Mg2+ (0.5 mM) folds the RNA. Reprinted with permission from Strulson, C. A., Yennawar, N. H., Rambo, R. P. & Bevilacqua, P. C. (2013). Molecular crowding favors reactivity of a human ribozyme under physiological ionic conditions. Biochemistry, 52, 8187–8197. Copyright 2016 American Chemical Society.