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. Author manuscript; available in PMC: 2017 Mar 28.
Published in final edited form as: Gene Ther. 2016 Sep 28;24(1):12–20. doi: 10.1038/gt.2016.69

Figure 1.

Figure 1

Integrase and LTR attachment site mutant vectors.(A) Integrase mutants derived upon mutation of the HIV-1 pol gene within the packaging plasmid, pMDL. Depicted are the locations of the c-terminal deletion and core domain point mutations. (B) LTR attachment site mutants derived upon deletion of the LTR attachment sites within the transfer vector, pcDNA-CS-CGW. Depicted are the locations of the 12 bp U3 (5’-ACTGGAAGGGCT-3’) and 11 bp U5 (5’-TCTCTAGCAGT-3’) attachment (att) site deletions. (C, D) Gag capsid protein (p24) was measured by ELISA in supernatants from 293T cells transfected with a GFP (C) or bleomycin resistance transgene containing transfer plasmid (D). Data is represented as mean ± SD.