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. Author manuscript; available in PMC: 2017 May 11.
Published in final edited form as: Gene Ther. 2016 Nov 11;24(1):49–59. doi: 10.1038/gt.2016.75

Fig. 4. Incubation of AAV vector with HSA pre-freezing or post-thawing of viruses has the similar enhanced effect.

Fig. 4

a. Enhanced transduction in Huh7 cells. 1×108 particles of AAV/luc were incubated with clinical grade HSA at different dilutions for 2hr at 4°C before virus freezing or after virus thawing, and then added to Huh7 cells. 48 hr later, luciferase activity in the cell lysate was measured. b and c. Enhanced muscle transduction. 1×109 particles of AAV8/luc were directly injected into muscles of mice. At day 7 post injection, the mouse imaging (b) was carried out (left panel) and the fold increase (c) of transgene expression was calculated (right panel, n=6). Face up: left leg-HSA, right leg-PBS.