Figure 3.

p11 in D2R-containing PrL neurons determines depression-like behavior. (a) Co-localization of dopamine D2 receptor (D2, red) and p11 (green) in layer II/III PrL neurons from D2-tdT (D2-Cre x tdTomato) mice. Scale bar, 10 μm. (b–e) Depression-like behaviors in wild-type (WT, p11^f/f) and D2⁺ neuron-specific conditional p11 knockout (p11 cKO, D2-Cre x p11^f/f) mice with the expression of AAV-DIO-p11 (AAV_p11) or AAV-DIO-eYFP (AAV_eYFP) in D2R-containing PrL neurons, as measured by TST (b), FST (c), SPT (d) and NSF (e) (n=6, WT+AAV_eYFP; n=6, WT+AAV_p11; n=12, p11 cKO+AAV_eYFP; n=12, p11 cKO+AAV_p11). (f) Immunofluorescence images of p11 (blue) and eYFP (green) in global p11 knockout (p11 gKO-tdT, D2-Cre x tdTomato x p11 KO) mice with the injection of AAV_p11 or AAV_eYFP into layer II/III PrL. Scale bar, 20 μm. (g–j) Depression-like behaviors in WT and p11 gKO (D2-Cre x p11 KO) mice with the injection of AAV_ p11 or AAV_eYFP into layer II/III PrL, as measured by TST (g), FST (h), SPT (i) and NSF (j) (n=15, WT; n=16, p11 gKO; n=7, p11 gKO+AAV_eYFP; n=6, p11 gKO+AAV_p11). *P<0.05, **P<0.01, one-way analysis of variance. Data are means±s.e.m. FST, forced swin test; NSF, novelty-supressed feeding; PrL, prelimbic cortex; SPT, sucrose preference test; TST, tail suspension test.

PowerPoint slide