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. 2017 Jan 27;7:36402. doi: 10.1038/srep36402

Figure 1. Pyrintegrin enhances adipogenesis of human adipose stem/progenitor cells in vitro.

Figure 1

(A) Ptn dose optimization by measuring PPARγ and C/EBPα expression in DMEM, AIM, AIM containing varying doses of Ptn 2-, 5- and 10-μM in hASC cultures after 4 days. Human GAPDH was used as the housekeeping gene and values representing mRNA of the DMEM group were defined as 1. AIM: adipogenesis induction medium; Ptn: Pyrintegrin. (B) Lipid accumulation in DMEM, AIM, AIM+Ptn 2-μM and Ptn 2-μM alone hASC cultures over 4 wks. Scale bar: 100 μm. (C) Relative mRNA expression of PPARγ and C/EBPα in DMEM, AIM, AIM+Ptn 2-μM and Ptn 2-μM alone hASC cultures at day 7. (D,E) Time-course real time RT-PCR analysis of PPARγ and C/EBPα expression of DMEM, AIM and AIM+Ptn 2-μM treated hASC cultures over 28 days. (F–I) Supernatant analysis of adiponectin, glycerol, leptin and triglycerides measured from DMEM, AIM and AIM+Ptn 2-μM treated hASC cultures at 2 and 4 wks. Data are expressed as mean ± S.D. *P < 0.05.