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. 2017 Jan 27;7:41166. doi: 10.1038/srep41166

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Copyright © 2017, The Author(s)

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(A) SGLT1, SGLT2, SGLT3, SGLT4, SGLT5, SGLT6 and SMIT1 detection by RT-PCR and ethidium bromide-stained agarose gels on mRNA extracted from non-failing human hearts (n = 4). Positive controls were intestine for SGLT1, kidney for SGLT2, SGLT3, SGLT4 and SGLT5, and brain for SGLT6 and SMIT1. mRNA copy numbers/μg of SGLT1 (B) and SMIT1 (C) RNA were measured in non-failing human hearts (n = 7) and compared to a positive control (n = 3). The clinical characteristics of patients are presented in Supplementary Table 1. (D) Comparison of SGLT1 and SMIT1 mRNA copy numbers/μg of RNA in human hearts (n = 7). Data were normalized to RPL32 and expressed as Log10 copy numbers/μg RNA. Data are means ± SEM. Statistical analysis was by Student’s t-test. *Indicates values statistically different from (C) corresponding control tissue (D) hSGLT1 mRNA expression, p ≤ 0.05.