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. 2017 Jan 27;7:40851. doi: 10.1038/srep40851

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Copyright © 2017, The Author(s)

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BY-T20 strain (BY4742, ΔTrp1, Trp1::HIS3-P_PGK1-BTS1/ERG20-T_ADH1-P_TDH3-SaGGPS-T_TPI1-P_TEF1-tHMG1-T_CYC1) containing different recombinant plasmid were cultivated in 100 mL liquid induction medium supplemented with 20 g/L galactose and grown at 30 °C for 3 d³⁶. (A) Accumulation of (E)-nerolidol; (B) Accumulation of (E,E)-geranyllinalool. pESC-Trp, BY-T20 yeast with pESC-Trp; pESC-Trp::TwNES, BY-T20 with pESC-Trp::TwNES recombinant plasmid; pESC-Trp::TwGES1, BY-T20 with pESC-Trp::TwGES1 recombinant plasmid. Values shown are means ± SD of six replicates (**P < 0.01).