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. 2017 Jan 27;7:41286. doi: 10.1038/srep41286

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Staining for key corneal proteins in mature corneal organoids. Inlay (i) represents the protein of interest, inlay (ii) is overlayed with F-actin (Red) and nuclei (blue) except in inlay K. Regions of interest highlighted with arrows, “*” denotes external surface of the organoid. (A–D) Demonstrates presence of corneal stromal proteins. (E–H) Demonstrates markers of basement membrane and epithelial differentiation. (I) Shows expression of P63α in apical cells, (J) shows dual immunostaining for P63α in red whilst the differentiated epithelial marker KRT3 in greenin the apical cells above the P63 positive cells (arrow). (K) Indicated the corneal crystallin ALDH3A1 being expressed in both the most apical cells and the underlying “stromal” cells.