Figure 1. Recombinant LFN-C3 is active: HeLa cell lysate was incubated with the purified recombinant fusion protein LFN-C3 in the presence of radio-labeled NAD⁺.

As negative control lysate was incubated with radio-labeled NAD⁺ without LFN-C3, as positive control with NAD⁺ plus Photorhabdus complete TccC3 ADP-ribosyltransferase (BC3). Shown is the readout of the dried SDS-PAGE as a typical result of three independent experiments.