Figure 2. Allergen exposure after ongoing M. tuberculosis infection results in the mixed expression of M1/M2 markers in lung.

BALB/c mice were infected with M. tuberculosis and then sensitized and challenged with OVA, as depicted in Fig. 1a. mRNA expression was measured by quantitative real-time PCR (a,b). The data represent the mean ± s.e.m. from 3 independent experiments (n = 4–9). Freshly cells from the lung were stained with the combination of surface markers, CD11c and CD206 and examined for flow cytometry. FSC^hi SSC^hi gate were used to detect CD11c and CD206 expression on cells (c). The data represent the mean ± s.e.m. (n = 6–7). TNF levels were evaluated in the lung homogenates (d). The data represent the mean ± s.e.m. from 2 independent experiments (n = 14–15) *p < 0.05.