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Editing and aminoacylation of TfeGly by wild-type IleRS (wt) and IleRSAla10 (10Ala): (a) AMP formation by 2 μM enzyme in the presence of 20 mM dl-TfeGly and in the absence of tRNAIle; (b) AMP formation by 2 μM enzyme in the presence of 20 mM dl-TfeGly and 10 μM tRNAIle; (c) single-turnover deacylation of preformed TfeGly-tRNAIle using 10 μM enzyme or no enzyme (nonenzymatic); (d) aminoacylation of tRNAIle with dl-TfeGly in the presence of 1 μM enzyme.
