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(a and b) Misaminoacylation of tRNAIle with TfeGly by 1 μM T243R/D342A IleRS in the presence or absence of activated EF-Tu on different time scales. (c) Misaminoacylation of tRNAIle with TfeGly by 2 μM wild-type IleRS in the presence or absence of activated EF-Tu. (d) Misaminoacylation and AMP formation by 5 μM T243R/D342A IleRS in the presence of TfeGly.
