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. 2016 Dec 23;3(1):73–80. doi: 10.1021/acscentsci.6b00339

Figure 6.

Figure 6

Effect of in vivo post-transfer editing of TfeGly. LC-ESI-MS experiment of VW18Ile variants expressed in the absence of Ile with supplementation of 1 mM TfeGly. For translation either the wild-type IleRS (a) or the post-transfer editing deficient mutant IleRSAla10 (b) was coexpressed. The monoisotopic masses of the 5-fold charged variants of the model peptide VW18Ile, that has just one Ile position, are displayed. Calculated monoisotopic masses for Ile substitution: Val = 605.78, Leu/Ile = 608.57, TfeGly = 613.75, Met = 612.19.