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. 2017 Jan 27;7:41612. doi: 10.1038/srep41612

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(A) Time course of LPS-induced activation of the WNT/β-catenin pathway. BEAS-2B human bronchial epithelial cells were stimulated with 0.1 μg/ml LPS for various time periods of 1–120 minutes. Western blotting was used to assess protein levels and phosphorylation of the members of the WNT/β-catenin pathway in total cell lysates. Beta-actin was used as endogenous control. (B) Suppressive effects of DKK-1 on LPS-induced activation of the WNT/β-catenin pathway. Cells were pretreated with 16–500 ng/ml of recombinant DKK-1 for 24 hours, followed by 0.1 μg/ml of LPS stimulation for 2 hours. (C,D) Suppressive effects of LGK974 on LPS-induced activation of the WNT/β-catenin pathway. Cells were pretreated with 10 nM LGK974 for 2 hours, followed by 0.1 μg/ml LPS stimulation for various time periods of 0.25–2 hours (C). Cells were pretreated with 0.001–10 nM of LGK974 for 2 hours, followed by 0.1 μg/ml of LPS stimulation for 2 hours (D).