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. 2017 Jan 27;7:41663. doi: 10.1038/srep41663

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Copyright © 2017, The Author(s)

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(a) Extracts from untreated MCF7 cells were precipitated with antibodies against B-Myb, pre-immune serum (pre) or antiserum against an unrelated protein (GST). Immunoprecipitates and aliquots of the total cell extract (TCE) were immunoblotted using antibodies against Rad50, Nbs1, Mre11 and B-Myb. (b) Cell extracts from untreated MCF7 cells were immunoprecipitated with antibodies against Rad50 or the Myc-tag. Immunoprecipitates were analyzed with antibodies against Rad50 and B-Myb. (c) QT6 fibroblasts were transfected with expression vectors for YFP, YFP-B-Myb, Mre11, Rad50 and Nbs1. Cell extracts were incubated with GFP-trap beads (containing a GFP-binding protein) and bound proteins (GFP-trap) as well as aliquots of the total cell extracts (TCE) were analyzed by western blotting with antibodies against Rad50, Nbs1, Mre11 and YFP.