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. 2016 Dec 28;6(1):288–297. doi: 10.1002/cam4.991

Figure 3.

Figure 3

HIF‐1 and CAIX affected the chemosensitivity of A549 cells under hypoxia. (A) Under normoxia, HIF‐1α 3A‐overexpressing A549 cells showed significant chemoresistance to vinorelbine in WST‐1 assay. (B) Stable HIF‐1α‐knockdown A549 cells, which were obtained by treatment with HIF‐1α shRNA vector, showed suppressed HIF‐1α expression compared with control cells. (C) Under hypoxia, HIF‐1α‐knockdown A549 cells showed more chemosensitivity to vinorelbine than control cells in WST‐1 assay. (D) CAIX‐knockdown A549 cells, which were transfected with CAIX siRNAs, showed suppressed CAIX expression compared with control cells. (E) Under hypoxia, CAIX‐knockdown cells showed more chemosensitivity to vinorelbine than control cells in WET‐1 assay. Best‐fit IC50 values were compared using the extra sum‐of‐square F test by using Prism 6 (**P < 0.001). Each value was standardized using that obtained for control cells. HIF‐1, hypoxia‐inducible factor 1; CAIX, carbonic anhydrase IX; WST1, 4‐[3‐(4‐iodophenyl)‐2‐(4‐nitrophenyl)‐2H‐5‐tetrazolio]‐1,3‐benzenedisulfonate.