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. 2017 Jan 27;7:41341. doi: 10.1038/srep41341

Figure 2. DnaK’s impact on E. coli growth is carbon source-dependent.

Figure 2

(A) Whole cell extracts of MG1655 wild type, ΔdnaKJ and rpoH(I54N) were separated on SDS-PAGE, stained with Coomassie Blue or analyzed by western blot using anti-Lon, -DnaK, -GroEL, -DnaJ or -RpoH antibodies. (B) Heat maps representing the averaged growth rates obtained from three different biological replicates of the three strains grown on 21 carbon sources. The blue scale indicates high (darker) to low (lighter) growth rate. Carbon sources were grouped into five classes each representing a different growth behavior of either the ΔdnaKJ or the rpoH(I54N) mutant compared to the wild type: class I groups carbon sources on which the two mutants did not grow, class II groups carbon sources on which both mutants exhibit higher growth rates than the wild type, class III groups carbon sources on which a growth defect of the dnaKJ mutant is observed, class IV groups carbon sources on which dnaKJ mutant exhibits a lowest rate of growth compared to both the wild-type strain and the rpoH(I54N) mutant and class V groups carbon sources on which no significant difference in growth was observed for both mutants and the wild type. Abbreviations NAG and NANA stand for N-acetyl-glucosamine and N-acetyl-neuraminate, respectively. (C) Representative growth curves of E. coli K-12 MG1655 wild type (green), ΔdnaKJ (red) and rpoH(I54N) (blue) for each class: Glucosamine (class I), Pyruvate (class II), Ribose (class III), Succinate (class IV) and Xylose (class V).