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. 2017 Jan 27;7:41428. doi: 10.1038/srep41428

Figure 1. Enzymatic activities of CCP5 splicing variants.

Figure 1

(a) Schematic representation of alternative splicing of Agbl5 that generates three CCP5 variants. DQ867034 and DQ867035 do not contain exon 4 and use the stop codons in exon 15 and 16 respectively. DQ867036 is the only CCP5 variant that contains exon 4 and uses the stop codon in exon 16. (b) When porcine tubulin was incubated with the lysate of HEK293 cells transfected with CCP5 variants, DQ867034 and DQ867035, it exhibited reduced GT335 immunoreactivity compared to LacZ transfected cells, indicative of active enzyme. In contrast, lysates from DQ867036 transfected cells showed no change in tubulin GT355 immunoreactivity, indicative of an inactive enzyme under these conditions. None of the 3 CCP5 variants altered polyE immunoreactivity, indicating their inability to cleave α-carboxyl–linked glutamate. (c) The activity of CCP5 (DQ867034) on branching glutamate (GT335) is inhibited by addition of 5 mM 1,10-phenanthroline (OP).