Figure 8. Abnormal mature sperm in Agbl5-KO mice.

(a–c) Hematoxylin-Eosin staining on sperm from epididymis and vas deferens of wild-type (a,a’), Agbl5-KO (b,b’), and pcd^3J (c) mice. The heads of sperm from Agbl5-KO mice are abnormally bent (b’, arrow) and segments of their tails are not ensheathed (b’, arrow head). Sperm from pcd^3J mice also have aberrant heads (c, arrow) and they frequently have multiple tails (c). (d–e) Mature sperm from epididymis and vas deferens of wild-type (d) and Agbl5-KO (e) mice are immunostained with α-tubulin antibody (ascites fluid B-5–12, green) and the nuclei visualized with DAPI (blue) staining. While α-tubulin antibody only stains the end piece of wild-type sperm (d, arrow), a large region in the middle of the tail of Agbl5-KO sperm is immunoreactive to the same antibody, indicating an unwrapped flagellum (e, arrow head). Scale bars: 10 μm in (a,b,c,d, and e); 5 μm in (a’ and b’).