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. 2017 Jan 27;7:41411. doi: 10.1038/srep41411

Figure 3. IL-6/sIL-6R differentially regulate resorption ability of varying concentration of RANKL-induced osteoclast.

Figure 3

(A) RAW264.7 cells were cultured with low level (10 ng/ml) or high level (50 ng/ml) of RANKL and M-CSF (30 ng/ml) in the presence or absence of IL-6/sIL-6R (100 ng/ml). After 14 days, cells were removed and the mineral-coated wells were counterstained by Von Kossa. Resorption pits were examined by light microscope. (B) The percentage of mineral-coated surface occupied by resorption lacunae from panels (A). (C) The quantification of the number of resorption pits from panels (A). (D) BMMs were cultured with low level (10 ng/ml) or high level (50 ng/ml) of RANKL and M-CSF (30 ng/ml) in the presence of IL-6/sIL-6R (100 ng/ml). After 14 days, cells were removed and the mineral-coated wells were counterstained by Von Kossa. Resorption pits were examined by light microscope. (E) The percentage of mineral-coated surface occupied by resorption lacunae from panels (D). (F) The quantification of the number of resorption pits from panels (D). Data are expressed as mean ± SD, (*p < 0.05, **p < 0.01).