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. 2017 Jan 27;7:41515. doi: 10.1038/srep41515

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(a) Epigallocatechin-3-gallate (EGCG). (b,c) ¹H,¹⁵N NMR correlation spectra of MAK33 V_L S20N and V_L P59A in presence and absence of EGCG. For S20N, addition of EGCG caused chemical shift perturbations for I58 and R61. Mutation of P59 prevented these shift changes. (d,e) Chemical shift perturbations for the S20N and P59A mutants in the presence of EGCG. Gaps indicate residues, which are not assigned.