Figure 2. PPARγ luciferase reporter assay.

Cos-7 cells were cotransfected with a PPARγ expression plasmid, a firefly luciferase gene under the control of three tandem PPARγ response elements, and a Renilla luciferase control. The firefly luciferase activity was normalized against Renilla luciferase units. Fold activation was calculated against DMSO vehicle. Fold activation of Rosi and compounds were at 0.1, 1 and 10 μM concentrations (n = 3, error bars = SEM).