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. 2017 Jan 27;7:41494. doi: 10.1038/srep41494

Figure 7. Immunostainings of lymphatic vessels and tumor cells in the draining sentinel LNs.

Figure 7

Draining sentinel LNs were resected from mice with either control ear sponges or sponges populated with B16F10Luc+ tumor cells. (a) Representative sections, belonging to the central LN region, are shown for control SLN and pre-metastatic SLN after 2 weeks of sponge insertion. (b) 3D reconstruction of the representative control and pre-metastatic SLN shown in (a), with lymphatic vessels in green and tissue border in blue. (c) Representative sections of control SLN and metastatic SLN, belonging to the central LN region, after 4 weeks of sponge implantation. (d) 3D reconstruction of the representative control and metastatic SLN shown in (c), with lymphatic vessels in green, tumor mass in red and tissue border in blue. Right panels in (a) and (c) represent higher magnification pictures of areas delineated by the square. Immunostained lymphatic vessels (LYVE-1 positive) appear in blue and tumor cells (tyrosinase positive) in pink. Black arrows indicate representative lymphatic vessels and the black dotted line delineate the tumor area. Scale bar represents 250 μm. For 3D reconstructions of SLN, around 40 sections were stacked, with a final z-stack thickness of ∼ 200 μm.