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. 2017 Jan 26;10:4. doi: 10.1186/s13072-017-0111-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — DNA methylation patterns of the developing fetal DLPFC. a Heatmaps of unsupervised hierarchical clustered DMPs found differentially methylated between early and late ST for each exposed and unexposed group. b Coronal sections from formalin-fixed, paraffin-embedded, previously frozen fetal cortex (20 wpc). Frontal region of cerebral hemisphere showing (i) hematoxylin and eosin (H&E) staining and (ii) NeuN staining, which distinctly labels the cortical plate (asterisk on H&E) and germinal matrix (arrowhead) (Scale = 2 mm). c CP estimates of NeuN⁻, NeuN⁺ and NPC within exposed and unexposed (local) fetal DLPFC samples (gray) were compared to CP estimates of publicly available postnatal frontal cortex generated by BrainSpan consortium (colored) (“Methods” section)