Figure 5.
Receptor tyrosine kinase inhibitors block infection only in cells that express high levels of the entry factor AXL. (A) The indicated cell lines were processed for immunoblot for AXL and the control tubulin. A representative experiment is shown. U2OS (B) or HBMEC (C) cells were treated with the indicated drugs and infected with ZIKV (Mex2-81, MOI=2.5) and processed for RT-qPCR analysis to monitor the level of infection from 3 independent experiments with mean±SD shown. (D–F) The indicated cell type was treated with the indicated drugs and infected with ZIKV (Mex2-81) and TCID50s were calculated for 3 independent experiments with mean±SD shown. *p,0.05.