Figure 3.

Eight-week-old Bmp6^fl/fl;Tek-Cre+ mice exhibit liver Bmp6 and Hamp deficiency and serum iron overload, whereas Bmp6^fl/fl;Alb-Cre+ and Bmp6^fl/fl;LysM-Cre+ mice do not. Eight-week-old littermate male (left) and female (right) Bmp6^+/+, Bmp6^+/−, and Bmp6^−/− global knockout mice (red bars) and Bmp6 conditional knockout mice in ECs (Bmp6^fl/fl;Tek-Cre+ [blue bars]), macrophages (Bmp6^fl/fl;LysM-Cre+ [green bars]), and hepatocytes (Bmp6^fl/fl;Alb-Cre+ [purple bars]) compared with littermate controls (Bmp6^fl/fl;Cre–) were analyzed for (A) total liver hepcidin (Hamp) and (D) Bmp6 relative to Rpl19 mRNA by qRT-PCR, (B) serum iron, and (C) transferrin (Tf) saturation. n = 4-11 mice per sex per group (supplemental Table 2). Results are reported as mean ± SEM of –ΔCt as in Figure 1. Bmp6 mRNA levels for Bmp6^+/+ and Bmp6^−/− mice, stratified by sex, were replotted from Figure 1 for comparison. *P < .05, **P < .01, ***P < .001 relative to control Bmp6^+/+ mice or Bmp6^fl/fl;Cre– mice by one-way ANOVA with Dunnett’s post hoc test or Student t test. nd, not detectable.