Table 1.

Primers used for this study

Primer	Sequencea	Reference
28S D2-3551 F	5’-CGG GTT GCT TGA GAG TGC AGC-3’	Mod. from Campbell et al. (2000)
28S D2-4039 R	5’-CTC CTT GGT CCG TGT TTC-3’	Mottern and Heraty (2014)
28S D3-4046 F	5’-TTG AAA CAC GGA CCA AGG AG-3’	Mod. from Nunn et al. (1996)
28S D3-4413 R	5’-TCG GAG GGA ACC AGC TAC TA-3’	Mod. from Nunn et al. (1996)
COI LCO-1490 F	5’-GGT CAA CAA ATC ATA AAG ATA TTG G-3’	Folmer et al. (1994)
COI HCO-2198 R	5’-TAA ACT TCA GGG TGA CCA AAA AAT CA-3’	Folmer et al. (1994)
COI NJ-2197 F	5’-TAT ATT TTA ATT YTW CCW GGA TTT GG-3’	Mod. from Simon et al. (1994)
COI MD-2614 R	5’-ATT GCA AAT ACT GCA CCT AT-3’	Dowton and Austin (1997)

^aNumbers following Ribosomal primers refer to the complimentary 5′ start position in Drosophila melanogaster (Tautz et al. 1988). Numbers following cytochrome oxidase subunit I primers refer to the complimentary 5′ start position in Drosophila yakuba (Folmer et al. 1994).