Table 2.
Primers used in reverse transcription PCR and quantitative RT-PCR
| Gene | Primers | Sequence(5′–3′) | Accession no. | |
|---|---|---|---|---|
| Reverse transcription PCR | ||||
| LsNAGA1 | F | CCTCAAATCAAGCAGACACG | KU757057 | |
| R | CCCATCCTTATGTTATTTTTCGTT | |||
| LsNAGA2 | F | CTTTGAAGATCGCCGTGAAT | KU757058 | |
| R | CCTCCATAAGTTGCAGTGCTC | |||
| LsCGT1 | F | TCCAGAATCTCCCAGCTACG | KU757055 | |
| R | CTGTCAACCCTCCCCAACTA | |||
| LsCGT2 | F | TGCCAAAAACTCACTATATCGAA | KU757056 | |
| R | CACAATGTTCCCAGCACTTTT | |||
| LsCK | F | AGCAACTTATTGTAAAGTTTCTCTTTG | KU757064 | |
| R | ATTTTCAATTACTTTACAGACAAAAGC | |||
| LsCS6 | F | CGTATCCACTACCGTTTGCAT | KU757063 | |
| R | GCGGCAAGTATCAATTCTAGTACA | |||
| LsGSC | F | GAACTAAGAATGCTTCTCCCACA | KU757062 | |
| R | GGCATCCAAGTTCAATGCTG | |||
| LsSMPD2 | F | CGTCTAGACCGGGCTTAAGAA | KU757061 | |
| R | GAACGATTGTTTACGACTGCAA | |||
| LsSPT | F | TGGAAGCGCCCTGTCTATTA | KU737582 | |
| R | AAAACAGTCTCTCTTCCGTTTCA | |||
| LsSMS | F | TATTTTCGAAGATGAAGCAAGC | KU757060 | |
| R | GGTGTTATTAATTCACCACCATTC | |||
| LsSMPD4 | F | CTCAACTACCATTGGGCATTC | KU757059 | |
| R | GCTCGCTGCGAAACAATAAG | |||
| LsSGPP | F | CCACAACGATGAAGGTGACT | KU757057 | |
| R | GACCATTGGCTGATCACTAGC | |||
| RSV-CP | F | CTAGTCATCTGCACCTTCTG | ||
| R | ATGGGTACCAACAAGCCAGC | |||
| Quantitative RT- PCR | ||||
| LsNAGA1 | F | GCTGAGTGGGAGGTCGATTA | ||
| R | CGCTGTAATTTGGCATCCTT | |||
| LsNAGA2 | F | ACAGGAGAACCGATGGTACG | ||
| R | GTAAGGGATGGGTTGCACAT | |||
| LsCGT1 | F | ACTGTTTGTGGGCAACAGTG | ||
| R | TCGCAAACAAACGGTAACTG | |||
| LsCGT2 | F | GTGGGAGGCATACAAGCATT | ||
| R | TCGCAACCCTTAATTTGGTC | |||
| LsCK | F | GCCAATCATGGGTATGAAGG | ||
| R | ACTTCTCGGACAGGCACAAG | |||
| LsCS6 | F | GTTGGCTCGCTTGTTCTTCT | ||
| R | ATCGCAGTGCTCCAAAGAAT | |||
| LsGSC | F | TGAAGGTTGCCATATCACCA | ||
| R | TAGGGATGCGTGGAAAAGTC | |||
| LsSMPD2 | F | TTGCGTTTGTGTCGAAAGAC | ||
| R | CCTCTATCGGCCATCTTGAA | |||
| LsSPT | F | TCCTAATGGGCACTTTCACC | ||
| R | CCATCTTGGCCCATAATGAC | |||
| LsSMS | F | CGGACACGAGCATTACTCAA | ||
| R | TGTCGTACTCGTTGGGAACA | |||
| LsSMPD4 | F | CCCCACTCCAAGACCTCATA | ||
| R | CACACAAGCGTGCTACCAAT | |||
| LsSGPP | F | GTCCTCCGGTACACAGGCTA | ||
| R | ATCACTAGGCCCAAATGCAG | |||
| LsActin | F | CCGCCTCCTCCAGTTCAC | ||
| R | TGTCCAC GTCGCACTTCAT | |||