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. 2016 Dec;11(12):1940–1950. doi: 10.4103/1673-5374.197135

Figure 5.

Figure 5

Validation of miRNA expression profiles by quantitative real time-PCR.

Validation of the expression profiles for rno-miR-206-3p, rno-miR-494-3p, rno-miR-6216 and rno-miR-3473 in the MCAO group at 24 hours vs. normal control (A), and rno-miR-494-3p in EA and SA groups vs. MCAO group at 24 hours (B), showing fold changes in microarray expression and quantitative real time-PCR using rno-U87 as a reference miRNA. Values are expressed as the mean ± SD. Statistical analysis was performed using unpaired t-test. MCAO: Middle cerebral artery occlusion; PCR: polymerase chain reaction; EA: electroacupuncture; SA: sham electroacupuncture.