Serial analysis of peripheral blood chimerism and lymphocyte composition after FCC HSCT. (A) Percentage donor chimerism of unfractionated, purified CD3 and purified CD15 peripheral blood cells. Mean and SEM are shown. (B) Reconstitution of peripheral blood lymphocytes after FCC HSCT. Median and interquartile range are shown, and the horizontal dotted lines enclosing gray boxes represent the median and interquartile range of 11 adult healthy volunteers. Comparisons between cell numbers at each time point and healthy volunteers were performed using a 2-tailed Mann-Whitney U test. Lymphocyte numbers at all time points were significantly below numbers for healthy volunteers (P < .0005). (C) Percentage of NK, B, and T cells. Mean and SEM are shown, and values for 11 adult healthy volunteers (HV) are indicated. (D) Percentage of CD4 and CD8 T cells within the CD3 T cell population using mean values. (E) Percentages of naïve, memory, and effector subset composition of CD4 T cells (left) and CD8 T cells (right). Mean and SEM are shown, and values for 11 adult healthy volunteers (HV) are indicated.