Figure 2.

Analysis of individual patients showed sustained mixed T cell chimerism at 1 year after FCC HSCT is due primarily to persistence of recipient CD8 T cells, with notable contribution of the effector subset. (A) Panels illustrate the gating strategy used for isolation of naïve, memory, and effector T cell subsets by FACS. (B) CD4 T cell (left) and CD8 T cell (right) subset chimerism at 1 year for 5 patients. CMV serostatus of donor/recipient and reactivation early post HSCT: patient 1, negative/negative; patient 2, negative/positive no reactivation; patient 3, positive/negative and reactivation; patient 4, negative/positive and reactivation; patient 5, negative/positive no reactivation.