UV-Visible analysis of the reaction between
NO2-CLA and thiols.
A, time course changes in absorbance at 330
(open circles), 290 (black
circles), and 250 nm (open
triangles) for the reaction between
NO2-CLA (10 μm) and GSH (3
mm). B, representative LC-UV-visible
traces of the reaction between purified 9-NO2-CLA
(100 μm) and BME (1.76 mm). Aliquots
were obtained at the indicated time points before LC-UV-visible
analysis. C, UV-visible spectra for
9-NO2-CLA (panel 1) and both
β- (panel 2) and δ-adducts
(panel 3). Asterisks
indicate minor peaks derived from contaminant
12-NO2-CLA.