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. 2016 Dec 6;292(4):1145–1159. doi: 10.1074/jbc.M116.756288

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FIGURE 3. — UV-Visible analysis of the reaction between NO₂-CLA and thiols. A, time course changes in absorbance at 330 (open circles), 290 (black circles), and 250 nm (open triangles) for the reaction between NO₂-CLA (10 μm) and GSH (3 mm). B, representative LC-UV-visible traces of the reaction between purified 9-NO₂-CLA (100 μm) and BME (1.76 mm). Aliquots were obtained at the indicated time points before LC-UV-visible analysis. C, UV-visible spectra for 9-NO₂-CLA (panel 1) and both β- (panel 2) and δ-adducts (panel 3). Asterisks indicate minor peaks derived from contaminant 12-NO₂-CLA.