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. 2016 Dec 6;292(4):1145–1159. doi: 10.1074/jbc.M116.756288

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FIGURE 4. — LC-MS/MS analysis of the reaction between NO₂-CLA and BME. A, purified 9-NO₂-CLA (10 μm) was reacted with BME (1.76 mm), and aliquots were obtained at 10 s (top), 5 min (middle), and 60 min (bottom) for analysis of free NO₂-CLA (right) and BME-NO₂-CLA adducts (left). B, representative time course for 9-NO₂-CLA reaction with BME. C and D, aliquots collected at the indicated times were incubated with NEM (100 mm), and the percentage of free NO₂-CLA with respect to that at time 0 (C) and the percentage of BME-NO₂-CLA adduct with respect to NEM-untreated controls (D) were determined. Data are representative of three independent experiments.