LC-MS/MS analysis of the reaction between
NO2-CLA and BME.
A, purified 9-NO2-CLA (10
μm) was reacted with BME (1.76 mm),
and aliquots were obtained at 10 s (top), 5 min
(middle), and 60 min
(bottom) for analysis of free
NO2-CLA (right) and
BME-NO2-CLA adducts (left).
B, representative time course for
9-NO2-CLA reaction with BME. C
and D, aliquots collected at the indicated
times were incubated with NEM (100 mm), and the
percentage of free NO2-CLA with respect to that at
time 0 (C) and the percentage of
BME-NO2-CLA adduct with respect to NEM-untreated
controls (D) were determined. Data are
representative of three independent experiments.